Conclusion Of The Study Of Standard Freezing And Thawing Protocols For Cryopreservation Of Biopsied Human Embryos At The Blastocyst Stage

In this study, we used standard protocol for blastocyst to freeze and thaw biopsied embryos at blastocyst stage. The freezing and thawing media are glycerol and sucrose respectively .Standard slow-freezing and rapid-thawing programs for blastocyst were performed. Our observation showed biopsied euploid, aneuploid and non-biopsied embryos have the similar survival rates and further development potential.The results show biopsied blastocyst stage embryos may not be affected by cryopreservation as the characteristics of blastocyst. From our observation, we also proved blastocysts with good quality have the similar survival rate and further development potential in vitro for euploid and aneuploid embryos .We postulate aneuploid blastocysts with good quality may not be affected by cryopreservation.

After PGD, human aneuploid embryos can be cultured to blastocyst stage and frozen as a test group to study cryopreservation for biopsied blastocyst stage embryos. In this study, there were more aneuploid blastocysts thawed than euploid blastocysts, because many euploid embryos were kept in frozen status until the FET cycles in need. In this situation, the aneuploid blastocysts are more valuable for cryopreservation study of biopsied embryos. We evaluate better survival blastocyst with >90% survival viable cells instead of intact blastocyst based on the blastocyst survival is more difficult to appraise, considering the number of cells and their specialization (22)However, good pregnancy and implantation rates are always obtained if blastocysts with >90% survival cells are replaced through our own experience.

From the present study, we concluded that post-thaw blastocyst survival and further development in vitro are similar between biopsied euploid, aneupolid and intact embryos .Clinical pregnancy and implantation rates are also similar between frozen embryo transfer cycles using biopsied embryos and those using intact embryos.

By: Anirban Bhattacharya

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